Immobilization of Glucoamylase on Macroporous Spheres
نویسندگان
چکیده
Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load, applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km value for immobilized glucoamylase was 1.28 % (w/v), pH and temperature optimum were 4.5 and 70°C, respectively. The conversion of 20 % (w/w) starch hydrolysate achieved with the immobilized glucoamylase was 97 % after 5 hours.
منابع مشابه
Temperature-Triggered Enzyme Immobilization and Release Based on Cross-Linked Gelatin Nanoparticles
A glucoamylase-immobilized system based on cross-linked gelatin nanoparticles (CLGNs) was prepared by coacervation method. This system exhibited characteristics of temperature-triggered phase transition, which could be used for enzyme immobilization and release. Their morphology and size distribution were examined by transmission electron microscopy and dynamic light scattering particle size an...
متن کاملPorous silica spheres in macroporous structures and on nanofibres.
Porous nanospheres have a wide range of applications such as in catalysis, separation and controlled delivery. Among these nanospheres, syntheses and applications of porous silica nanospheres have been investigated extensively. Uniform porous silica nanospheres can be synthesized using a modified Stöber method. In the present study, porous silica spheres were prepared in the pre-formed emulsion...
متن کاملImmobilization of denatured DNA to macroporous supports: I. Efficiency of different coupling procedures.
Methods commonly used for covalent immobilization of single stranded DNA have been applied to several solid supports (Sephadex G-25 and Cellex 410) as well as to a number of macroporous materials (Sepharose C1-6B, C1-2B; Sephacryl S-500 and S-1000). Coupling efficiencies and stability of covalently bound DNA are compared for both classes of materials. The yields of the immobilization reaction f...
متن کاملFacile Synthesis of Radial-Like Macroporous Superparamagnetic Chitosan Spheres with In-Situ Co-Precipitation and Gelation of Ferro-Gels
Macroporous chitosan spheres encapsulating superparamagnetic iron oxide nanoparticles were synthesized by a facile and effective one-step fabrication process. Ferro-gels containing ferrous cations, ferric cations and chitosan were dropped into a sodium hydroxide solution through a syringe pump. In addition, a sodium hydroxide solution was employed for both gelation (chitosan) and co-precipitati...
متن کاملThree-dimensionally ordered macroporous carbons having walls composed of hollow mesosized spheres.
Three-dimensionally ordered macroporous carbons were prepared from bimodal polymer-silica colloidal crystals; the resulting carbons had interconnected macropores, and the walls of the macropores were composed of hollow mesosized spheres.
متن کامل